4RS6

Crystal structure of the C domain of Polo like Kinase II in Homo Sapiens

4RS6 の概要

• エントリーDOI: 10.2210/pdb4rs6/pdb
• 分子名称: Serine/threonine-protein kinase PLK2 (2 entities in total)
• 機能のキーワード: first pbd domain of polo like kinase ii, phosphorylation of target protein, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole : Q9NYY3
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54888.18

構造登録者

Shan, H.,Quan, J.,Wang, T. (登録日: 2014-11-07, 公開日: 2014-12-03, 最終更新日: 2023-11-08)

主引用文献

Shan, H.M.,Wang, T.,Quan, J.M.
Crystal structure of the polo-box domain of polo-like kinase 2
Biochem.Biophys.Res.Commun., 456:780-784, 2015

PubMed Abstract: Polo-like kinase 2 (PLK2) is a crucial regulator in cell cycle progression, DNA damage response, and neuronal activity. PLK2 is characterized by the conserved N-terminal kinase domain and the unique C-terminal polo-box domain (PBD). The PBD mediates diverse functions of PLK2 by binding phosphorylated Ser-pSer/pThr motifs of its substrates. Here, we report the first crystal structure of the PBD of PLK2. The overall structure of the PLK2 PBD is similar to that of the PLK1 PBD, which is composed by two polo boxes each contain β6α structures that form a 12-stranded β sandwich domain. The edge of the interface between the two polo boxes forms the phosphorylated Ser-pSer/pThr motifs binding cleft. On the hand, the peripheral regions around the core binding cleft of the PLK2 PBD is distinct from that of the PLK1 PBD, which might confer the substrate specificity of the PBDs of the polo-like kinase family.

PubMed: 25511705
DOI: 10.1016/j.bbrc.2014.11.125

実験手法

X-RAY DIFFRACTION (2.6 Å)