4RNP

BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY

「3RNP」から置き換えられました 「2RNP」から置き換えられました

4RNP の概要

• エントリーDOI: 10.2210/pdb4rnp/pdb
• 分子名称: RNA POLYMERASE (1 entity in total)
• 機能のキーワード: nucleotidyltransferase, rna polymerase, transcription
• 由来する生物種: Enterobacteria phage T7
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 296952.68

構造登録者

Liu, Z.J.,Sousa, R.,Rose, J.P.,Wang, B.C. (登録日: 1997-09-11, 公開日: 1997-12-03, 最終更新日: 2024-02-28)

主引用文献

Sousa, R.,Chung, Y.J.,Rose, J.P.,Wang, B.C.
Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution.
Nature, 364:593-599, 1993

PubMed Abstract: The crystal structure of T7 RNA polymerase reveals a molecule organized around a cleft that can accommodate a double-stranded DNA template. A portion (approximately 45%) of the molecule displays extensive structural homology to the polymerase domain of Klenow fragment and more limited homology to the human immunodeficiency virus HIV-1 reverse transcriptase. A comparison of the structures and sequences of these polymerases identifies structural elements that may be responsible for discriminating between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA templates. The relative locations of the catalytic site and a specific promoter recognition residue allow the orientation of the polymerase on the template to be defined.

PubMed: 7688864
DOI: 10.1038/364593a0

実験手法

X-RAY DIFFRACTION (3 Å)