4RKG

Structure of the MSL2 CXC domain bound with a non-specific (GC)6 DNA

4RKG の概要

• エントリーDOI: 10.2210/pdb4rkg/pdb
• 関連するPDBエントリー: 4RKH
• 分子名称: E3 ubiquitin-protein ligase msl-2, DNA (5'-D(*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*C)-3'), ZINC ION (3 entities in total)
• 機能のキーワード: zinc cluster, dna binding domain, dosage compensation, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• 細胞内の位置: Nucleus: P50534
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 19224.42

構造登録者

Zheng, S.,Ye, K. (登録日: 2014-10-13, 公開日: 2015-01-21, 最終更新日: 2024-05-01)

主引用文献

Zheng, S.,Villa, R.,Wang, J.,Feng, Y.,Wang, J.,Becker, P.B.,Ye, K.
Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation.
Genes Dev., 28:2652-2662, 2014

PubMed Abstract: The male-specific lethal dosage compensation complex (MSL-DCC) selectively assembles on the X chromosome in Drosophila males and activates gene transcription by twofold through histone acetylation. An MSL recognition element (MRE) sequence motif nucleates the initial MSL association, but how it is recognized remains unknown. Here, we identified the CXC domain of MSL2 specifically recognizing the MRE motif and determined its crystal structure bound to specific and nonspecific DNAs. The CXC domain primarily contacts one strand of DNA duplex and employs a single arginine to directly read out dinucleotide sequences from the minor groove. The arginine is flexible when bound to nonspecific sequences. The core region of the MRE motif harbors two binding sites on opposite strands that can cooperatively recruit a CXC dimer. Specific DNA-binding mutants of MSL2 are impaired in MRE binding and X chromosome localization in vivo. Our results reveal multiple dynamic DNA-binding modes of the CXC domain that target the MSL-DCC to X chromosomes.

PubMed: 25452275
DOI: 10.1101/gad.250936.114

実験手法

X-RAY DIFFRACTION (2.5 Å)