4RKF

Drosophila melanogaster Rab3 bound to GMPPNP

4RKF の概要

• エントリーDOI: 10.2210/pdb4rkf/pdb
• 関連するPDBエントリー: 1ZBD 3RAB 4RKE
• 分子名称: Ras-related protein Rab-3, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: gtp hydrolysis, hydrolase
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle: P25228
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45567.18

構造登録者

Lardong, J.A.,Driller, J.H.,Depner, H.,Weise, C.,Petzoldt, A.,Wahl, M.C.,Sigrist, S.J.,Loll, B. (登録日: 2014-10-13, 公開日: 2014-12-10, 最終更新日: 2023-09-20)

主引用文献

Lardong, J.A.,Driller, J.H.,Depner, H.,Weise, C.,Petzoldt, A.,Wahl, M.C.,Sigrist, S.J.,Loll, B.
Structures of Drosophila melanogaster Rab2 and Rab3 bound to GMPPNP.
Acta Crystallogr F Struct Biol Commun, 71:34-40, 2015

PubMed Abstract: Rab GTPases belong to the large family of Ras proteins. They act as key regulators of membrane organization and intracellular trafficking. Functionally, they act as switches. In the active GTP-bound form they can bind to effector proteins to facilitate the delivery of transport vesicles. Upon stimulation, the GTP is hydrolyzed and the Rab proteins undergo conformational changes in their switch regions. This study focuses on Rab2 and Rab3 from Drosophila melanogaster. Whereas Rab2 is involved in vesicle transport between the Golgi and the endoplasmatic reticulum, Rab3 is a key player in exocytosis, and in the synapse it is involved in the assembly of the presynaptic active zone. Here, high-resolution crystal structures of Rab2 and Rab3 in complex with GMPPNP and Mg2+ are presented. In the structure of Rab3 a modified cysteine residue is observed with an enigmatic electron density attached to its thiol function.

PubMed: 25615965
DOI: 10.1107/S2053230X1402617X

実験手法

X-RAY DIFFRACTION (1.5 Å)