4RGQ

Crystal structure of the Methanocaldococcus jannaschii G1PDH with NADPH and DHAP

4RGQ の概要

• エントリーDOI: 10.2210/pdb4rgq/pdb
• 関連するPDBエントリー: 4RFL 4RGV
• 分子名称: Glycerol-1-phosphate dehydrogenase, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, POTASSIUM ION, ... (8 entities in total)
• 機能のキーワード: dehydrogenase, oxidoreductase, rossman fold, metal ion binding, nadp(h) binding, zn, sn-glycerol-1-phosphate dehydrogenase
• 由来する生物種: Methanocaldococcus jannaschii
• 細胞内の位置: Cytoplasm : Q58122
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 169731.16

構造登録者

Carbone, V.,Ronimus, R.S.,Schofield, L.R.,Sutherland-Smith, A.J. (登録日: 2014-09-30, 公開日: 2015-07-22, 最終更新日: 2023-09-20)

主引用文献

Carbone, V.,Schofield, L.R.,Zhang, Y.,Sang, C.,Dey, D.,Hannus, I.M.,Martin, W.F.,Sutherland-Smith, A.J.,Ronimus, R.S.
Structure and Evolution of the Archaeal Lipid Synthesis Enzyme sn-Glycerol-1-phosphate Dehydrogenase.
J.Biol.Chem., 290:21690-21704, 2015

PubMed Abstract: One of the most critical events in the origins of cellular life was the development of lipid membranes. Archaea use isoprenoid chains linked via ether bonds to sn-glycerol 1-phosphate (G1P), whereas bacteria and eukaryotes use fatty acids attached via ester bonds to enantiomeric sn-glycerol 3-phosphate. NAD(P)H-dependent G1P dehydrogenase (G1PDH) forms G1P and has been proposed to have played a crucial role in the speciation of the Archaea. We present here, to our knowledge, the first structures of archaeal G1PDH from the hyperthermophilic methanogen Methanocaldococcus jannaschii with bound substrate dihydroxyacetone phosphate, product G1P, NADPH, and Zn(2+) cofactor. We also biochemically characterized the enzyme with respect to pH optimum, cation specificity, and kinetic parameters for dihydroxyacetone phosphate and NAD(P)H. The structures provide key evidence for the reaction mechanism in the stereospecific addition for the NAD(P)H-based pro-R hydrogen transfer and the coordination of the Zn(2+) cofactor during catalysis. Structure-based phylogenetic analyses also provide insight into the origins of G1PDH.

PubMed: 26175150
DOI: 10.1074/jbc.M115.647461

実験手法

X-RAY DIFFRACTION (2.23 Å)