4RGF

Crystal structure of the in-line aligned env22 twister ribozyme soaked with Mn2+

4RGF の概要

• エントリーDOI: 10.2210/pdb4rgf/pdb
• 関連するPDBエントリー: 4RGE
• 分子名称: env22 twister ribozyme, MANGANESE (II) ION, POTASSIUM ION, ... (5 entities in total)
• 機能のキーワード: pseudoknot, stems, cleavage, rna
• 由来する生物種: Synthetic
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 55208.26

構造登録者

Ren, A.,Rajashankar, K.R.,Simanshu, D.,Patel, D. (登録日: 2014-09-30, 公開日: 2014-12-03, 最終更新日: 2024-02-28)

主引用文献

Ren, A.,Kosutic, M.,Rajashankar, K.R.,Frener, M.,Santner, T.,Westhof, E.,Micura, R.,Patel, D.J.
In-line alignment and Mg(2+) coordination at the cleavage site of the env22 twister ribozyme.
Nat Commun, 5:5534-5534, 2014

PubMed Abstract: Small self-cleaving nucleolytic ribozymes contain catalytic domains that accelerate site-specific cleavage/ligation of phosphodiester backbones. We report on the 2.9-Å crystal structure of the env22 twister ribozyme, which adopts a compact tertiary fold stabilized by co-helical stacking, double-pseudoknot formation and long-range pairing interactions. The U-A cleavage site adopts a splayed-apart conformation with the modelled 2'-O of U positioned for in-line attack on the adjacent to-be-cleaved P-O5' bond. Both an invariant guanosine and a Mg(2+) are directly coordinated to the non-bridging phosphate oxygens at the U-A cleavage step, with the former positioned to contribute to catalysis and the latter to structural integrity. The impact of key mutations on cleavage activity identified an invariant guanosine that contributes to catalysis. Our structure of the in-line aligned env22 twister ribozyme is compared with two recently reported twister ribozymes structures, which adopt similar global folds, but differ in conformational features around the cleavage site.

PubMed: 25410397
DOI: 10.1038/ncomms6534

実験手法

X-RAY DIFFRACTION (3.2008 Å)