4REX

Crystal structure of the first WW domain of human YAP2 isoform

4REX の概要

• エントリーDOI: 10.2210/pdb4rex/pdb
• 分子名称: Yorkie homolog, SULFATE ION (3 entities in total)
• 機能のキーワード: ww domain, hippo signaling pathway, antiparallel beta-sheet, protein binding, proline rich motifs
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P46937
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5625.24

構造登録者

Camara-Artigas, A. (登録日: 2014-09-24, 公開日: 2015-08-19, 最終更新日: 2023-09-20)

主引用文献

Martinez-Rodriguez, S.,Bacarizo, J.,Luque, I.,Camara-Artigas, A.
Crystal structure of the first WW domain of human YAP2 isoform.
J.Struct.Biol., 191:381-387, 2015

PubMed Abstract: The WW domains are the smallest modular domains known. The study of the structural basis of their stability is important to understand their physiological role. These domains are intrinsically flexible, which makes them difficult to crystallize. The first WW domain of the human Yes tyrosine kinase Associated Protein (YAP) has been crystallized and its structure has been solved by X-ray diffraction at 1.6 Å resolution. Crystals belong to the orthorhombic space group P21212 with unit cell parameters a=42.67, b=43.10 and c=21.30. The addition of proline and other small-molecule additives improves drastically the quality of the crystals. The interactions that stabilize this minimal modular domain have been analysed. This crystal structure reveals that, besides the stabilization of the hydrophobic core of the protein by the aromatic cluster formed by Trp177-Phe189-Pro202, some salt-bridges interactions might affect the stability of the domain.

PubMed: 26256245
DOI: 10.1016/j.jsb.2015.08.001

実験手法

X-RAY DIFFRACTION (1.6 Å)