4REV

Structure of the dirigent protein DRR206

4REV の概要

• エントリーDOI: 10.2210/pdb4rev/pdb
• 分子名称: Disease resistance response protein 206, GLYCEROL, 2,3-DIMETHYLIMIDAZOLIUM ION, ... (5 entities in total)
• 機能のキーワード: beta-barrel, stereoselective coupling, coniferyl alcohol, plant protein
• 由来する生物種: Pisum sativum (garden pea,peas)
• 細胞内の位置: Secreted, extracellular space, apoplast : P13240
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36825.48

構造登録者

Kim, K.-Y.,Smith, C.A.,Merkley, E.D.,Cort, J.R.,Davin, L.B.,Lewis, N.G. (登録日: 2014-09-24, 公開日: 2014-11-26, 最終更新日: 2024-11-06)

主引用文献

Kim, K.W.,Smith, C.A.,Daily, M.D.,Cort, J.R.,Davin, L.B.,Lewis, N.G.
Trimeric Structure of (+)-Pinoresinol-forming Dirigent Protein at 1.95 angstrom Resolution with Three Isolated Active Sites.
J.Biol.Chem., 290:1308-1318, 2015

PubMed Abstract: Control over phenoxy radical-radical coupling reactions in vivo in vascular plants was enigmatic until our discovery of dirigent proteins (DPs, from the Latin dirigere, to guide or align). The first three-dimensional structure of a DP ((+)-pinoresinol-forming DP, 1.95 Å resolution, rhombohedral space group H32)) is reported herein. It has a tightly packed trimeric structure with an eight-stranded β-barrel topology for each DP monomer. Each putative substrate binding and orientation coupling site is located on the trimer surface but too far apart for intermolecular coupling between sites. It is proposed that each site enables stereoselective coupling (using either two coniferyl alcohol radicals or a radical and a monolignol). Interestingly, there are six differentially conserved residues in DPs affording either the (+)- or (-)-antipodes in the vicinity of the putative binding site and region known to control stereoselectivity. DPs are involved in lignan biosynthesis, whereas dirigent domains/sites have been implicated in lignin deposition.

PubMed: 25411250
DOI: 10.1074/jbc.M114.611780

実験手法

X-RAY DIFFRACTION (1.95 Å)