4R8U

S-SAD structure of DINB-DNA Complex

4R8U の概要

• エントリーDOI: 10.2210/pdb4r8u/pdb
• 分子名称: DNA polymerase IV, DNA, MAGNESIUM ION, ... (8 entities in total)
• 機能のキーワード: nucleotidyl transferase, dna polymerase, transferase-dna complex, transferase/dna
• 由来する生物種: Escherichia coli K-12; 詳細
• 細胞内の位置: Cytoplasm : Q47155 Q47155
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 98111.83

構造登録者

Kottur, J.,Nair, D.T.,Weinert, T.,Oligeric, V.,Wang, M. (登録日: 2014-09-03, 公開日: 2015-01-14, 最終更新日: 2024-03-20)

主引用文献

Weinert, T.,Olieric, V.,Waltersperger, S.,Panepucci, E.,Chen, L.,Zhang, H.,Zhou, D.,Rose, J.,Ebihara, A.,Kuramitsu, S.,Li, D.,Howe, N.,Schnapp, G.,Pautsch, A.,Bargsten, K.,Prota, A.E.,Surana, P.,Kottur, J.,Nair, D.T.,Basilico, F.,Cecatiello, V.,Pasqualato, S.,Boland, A.,Weichenrieder, O.,Wang, B.C.,Steinmetz, M.O.,Caffrey, M.,Wang, M.
Fast native-SAD phasing for routine macromolecular structure determination
Nat.Methods, 12:131-133, 2015

PubMed Abstract: We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.

PubMed: 25506719
DOI: 10.1038/nmeth.3211

実験手法

X-RAY DIFFRACTION (2.3 Å)