4R70

Crystal structure of bacteriophytochrome RpBphP3 from photosynthetic bacterium R. palustris

4R70 の概要

• エントリーDOI: 10.2210/pdb4r70/pdb
• 関連するPDBエントリー: 2OOL 4R6L
• 分子名称: Bacteriophytochrome (Light-regulated signal transduction histidine kinase), PhyB2, BILIVERDINE IX ALPHA (3 entities in total)
• 機能のキーワード: pas fold, photoreceptor, response regulator rpa3017, transferase, signaling protein
• 由来する生物種: Rhodopseudomonas palustris CGA009
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 118806.57

構造登録者

Yang, X.,Kuk, J.,Moffat, K. (登録日: 2014-08-26, 公開日: 2015-07-15, 最終更新日: 2024-11-20)

主引用文献

Yang, X.,Stojkovic, E.A.,Ozarowski, W.B.,Kuk, J.,Davydova, E.,Moffat, K.
Light Signaling Mechanism of Two Tandem Bacteriophytochromes.
Structure, 23:1179-1189, 2015

PubMed Abstract: RpBphP2 and RpBphP3, two tandem bacteriophytochromes from the photosynthetic bacterium Rhodopseudomonas palustris, share high sequence identity but exhibit distinct photoconversion behavior. Unlike the canonical RpBphP2, RpBphP3 photoconverts to an unusual near-red-absorbing (Pnr) state; both are required for synthesis of light-harvesting complexes under low-light conditions. Here we report the crystal structures of the photosensory core modules of RpBphP2 and RpBphP3. Despite different quaternary structures, RpBphP2 and RpBphP3 adopt nearly identical tertiary structures. The RpBphP3 structure reveals tongue-and-groove interactions at the interface between the GAF and PHY domains. A single mutation in the PRxSF motif at the GAF-PHY interface abolishes light-induced formation of the Pnr state in RpBphP3, possibly due to altered structural rigidity of the chromophore-binding pocket. Structural comparisons suggest that long-range signaling involves structural rearrangement of the helical spine at the dimer interface. These structures, together with mutational studies, provide insights into photoconversion and the long-range signaling mechanism in phytochromes.

PubMed: 26095026
DOI: 10.1016/j.str.2015.04.022

実験手法

X-RAY DIFFRACTION (2.85 Å)