4R3A

Erythrobacter litoralis EL346 blue-light activated histidine kinase

Summary for 4R3A

• Entry DOI: 10.2210/pdb4r3a/pdb
• Related: 4R38 4R39
• Descriptor: Blue-light-activated histidine kinase 2, MAGNESIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (4 entities in total)
• Functional Keywords: light-activated, lov domain, histidine kinase, bergerat fold, signal transduction, sensory transduction, photoreceptor, cell signaling, regulation, two-component system, signaling protein
• Biological source: Erythrobacter litoralis HTCC2594
• Total number of polymer chains: 2
• Total formula weight: 79058.43

Authors

Tomchick, D.R.,Rivera-Cancel, G.,Gardner, K.H. (deposition date: 2014-08-14, release date: 2014-12-03, Last modification date: 2024-10-16)

Primary citation

Rivera-Cancel, G.,Ko, W.H.,Tomchick, D.R.,Correa, F.,Gardner, K.H.
Full-length structure of a monomeric histidine kinase reveals basis for sensory regulation.
Proc.Natl.Acad.Sci.USA, 111:17839-17844, 2014

PubMed Abstract: Although histidine kinases (HKs) are critical sensors of external stimuli in prokaryotes, the mechanisms by which their sensor domains control enzymatic activity remain unclear. Here, we report the full-length structure of a blue light-activated HK from Erythrobacter litoralis HTCC2594 (EL346) and the results of biochemical and biophysical studies that explain how it is activated by light. Contrary to the standard view that signaling occurs within HK dimers, EL346 functions as a monomer. Its structure reveals that the light-oxygen-voltage (LOV) sensor domain both controls kinase activity and prevents dimerization by binding one side of a dimerization/histidine phosphotransfer-like (DHpL) domain. The DHpL domain also contacts the catalytic/ATP-binding (CA) domain, keeping EL346 in an inhibited conformation in the dark. Upon light stimulation, interdomain interactions weaken to facilitate activation. Our data suggest that the LOV domain controls kinase activity by affecting the stability of the DHpL/CA interface, releasing the CA domain from an inhibited conformation upon photoactivation. We suggest parallels between EL346 and dimeric HKs, with sensor-induced movements in the DHp similarly remodeling the DHp/CA interface as part of activation.

PubMed: 25468971
DOI: 10.1073/pnas.1413983111

Experimental method

X-RAY DIFFRACTION (2.92 Å)