4R3A

Erythrobacter litoralis EL346 blue-light activated histidine kinase

4R3A の概要

• エントリーDOI: 10.2210/pdb4r3a/pdb
• 関連するPDBエントリー: 4R38 4R39
• 分子名称: Blue-light-activated histidine kinase 2, MAGNESIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (4 entities in total)
• 機能のキーワード: light-activated, lov domain, histidine kinase, bergerat fold, signal transduction, sensory transduction, photoreceptor, cell signaling, regulation, two-component system, signaling protein
• 由来する生物種: Erythrobacter litoralis HTCC2594
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79058.43

構造登録者

Tomchick, D.R.,Rivera-Cancel, G.,Gardner, K.H. (登録日: 2014-08-14, 公開日: 2014-12-03, 最終更新日: 2024-10-16)

主引用文献

Rivera-Cancel, G.,Ko, W.H.,Tomchick, D.R.,Correa, F.,Gardner, K.H.
Full-length structure of a monomeric histidine kinase reveals basis for sensory regulation.
Proc.Natl.Acad.Sci.USA, 111:17839-17844, 2014

PubMed Abstract: Although histidine kinases (HKs) are critical sensors of external stimuli in prokaryotes, the mechanisms by which their sensor domains control enzymatic activity remain unclear. Here, we report the full-length structure of a blue light-activated HK from Erythrobacter litoralis HTCC2594 (EL346) and the results of biochemical and biophysical studies that explain how it is activated by light. Contrary to the standard view that signaling occurs within HK dimers, EL346 functions as a monomer. Its structure reveals that the light-oxygen-voltage (LOV) sensor domain both controls kinase activity and prevents dimerization by binding one side of a dimerization/histidine phosphotransfer-like (DHpL) domain. The DHpL domain also contacts the catalytic/ATP-binding (CA) domain, keeping EL346 in an inhibited conformation in the dark. Upon light stimulation, interdomain interactions weaken to facilitate activation. Our data suggest that the LOV domain controls kinase activity by affecting the stability of the DHpL/CA interface, releasing the CA domain from an inhibited conformation upon photoactivation. We suggest parallels between EL346 and dimeric HKs, with sensor-induced movements in the DHp similarly remodeling the DHp/CA interface as part of activation.

PubMed: 25468971
DOI: 10.1073/pnas.1413983111

実験手法

X-RAY DIFFRACTION (2.92 Å)