4R0W

Vvtgvta, an amyloid forming segment from alpha synuclein, residues 70-76

4R0W の概要

• エントリーDOI: 10.2210/pdb4r0w/pdb
• 関連するPDBエントリー: 4r0u
• 分子名称: Alpha-synuclein (2 entities in total)
• 機能のキーワード: amyloid-like protofibril, protein fibril
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P37840
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 645.75

構造登録者

Ivanova, M.I.,Eisenberg, D.S.,Sawaya, M.R. (登録日: 2014-08-01, 公開日: 2014-12-17, 最終更新日: 2024-04-03)

主引用文献

Li, D.,Jones, E.M.,Sawaya, M.R.,Furukawa, H.,Luo, F.,Ivanova, M.,Sievers, S.A.,Wang, W.,Yaghi, O.M.,Liu, C.,Eisenberg, D.S.
Structure-based design of functional amyloid materials.
J.Am.Chem.Soc., 136:18044-18051, 2014

PubMed Abstract: Amyloid fibers, once exclusively associated with disease, are acquiring utility as a class of biological nanomaterials. Here we introduce a method that utilizes the atomic structures of amyloid peptides, to design materials with versatile applications. As a model application, we designed amyloid fibers capable of capturing carbon dioxide from flue gas, to address the global problem of excess anthropogenic carbon dioxide. By measuring dynamic separation of carbon dioxide from nitrogen, we show that fibers with designed amino acid sequences double the carbon dioxide binding capacity of the previously reported fiber formed by VQIVYK from Tau protein. In a second application, we designed fibers that facilitate retroviral gene transfer. By measuring lentiviral transduction, we show that designed fibers exceed the efficiency of polybrene, a commonly used enhancer of transduction. The same procedures can be adapted to the design of countless other amyloid materials with a variety of properties and uses.

PubMed: 25474758
DOI: 10.1021/ja509648u

実験手法

X-RAY DIFFRACTION (1.5 Å)