4QYZ
Crystal structure of a CRISPR RNA-guided surveillance complex, Cascade, bound to a ssDNA target
Summary for 4QYZ
| Entry DOI | 10.2210/pdb4qyz/pdb |
| Descriptor | CRISPR system Cascade subunit CasA, CRISPR system Cascade subunit CasB, CRISPR system Cascade subunit CasC, ... (8 entities in total) |
| Functional Keywords | crispr-associated, bacterial immunity, cas3, immune system-dna-rna complex, immune system/dna/rna |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 13 |
| Total formula weight | 413400.26 |
| Authors | Mulepati, S.,Bailey, S. (deposition date: 2014-07-26, release date: 2014-09-03, Last modification date: 2014-10-01) |
| Primary citation | Mulepati, S.,Heroux, A.,Bailey, S. Structural biology. Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target. Science, 345:1479-1484, 2014 Cited by PubMed Abstract: In prokaryotes, RNA derived from type I and type III CRISPR loci direct large ribonucleoprotein complexes to destroy invading bacteriophage and plasmids. In Escherichia coli, this 405-kilodalton complex is called Cascade. We report the crystal structure of Cascade bound to a single-stranded DNA (ssDNA) target at a resolution of 3.03 angstroms. The structure reveals that the CRISPR RNA and target strands do not form a double helix but instead adopt an underwound ribbon-like structure. This noncanonical structure is facilitated by rotation of every sixth nucleotide out of the RNA-DNA hybrid and is stabilized by the highly interlocked organization of protein subunits. These studies provide insight into both the assembly and the activity of this complex and suggest a mechanism to enforce fidelity of target binding. PubMed: 25123481DOI: 10.1126/science.1256996 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.0303 Å) |
Structure validation
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