4QYZ

Crystal structure of a CRISPR RNA-guided surveillance complex, Cascade, bound to a ssDNA target

4QYZ の概要

• エントリーDOI: 10.2210/pdb4qyz/pdb
• 分子名称: CRISPR system Cascade subunit CasA, CRISPR system Cascade subunit CasB, CRISPR system Cascade subunit CasC, ... (8 entities in total)
• 機能のキーワード: crispr-associated, bacterial immunity, cas3, immune system-dna-rna complex, immune system/dna/rna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 413400.26

構造登録者

Mulepati, S.,Bailey, S. (登録日: 2014-07-26, 公開日: 2014-09-03, 最終更新日: 2014-10-01)

主引用文献

Mulepati, S.,Heroux, A.,Bailey, S.
Structural biology. Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target.
Science, 345:1479-1484, 2014

PubMed Abstract: In prokaryotes, RNA derived from type I and type III CRISPR loci direct large ribonucleoprotein complexes to destroy invading bacteriophage and plasmids. In Escherichia coli, this 405-kilodalton complex is called Cascade. We report the crystal structure of Cascade bound to a single-stranded DNA (ssDNA) target at a resolution of 3.03 angstroms. The structure reveals that the CRISPR RNA and target strands do not form a double helix but instead adopt an underwound ribbon-like structure. This noncanonical structure is facilitated by rotation of every sixth nucleotide out of the RNA-DNA hybrid and is stabilized by the highly interlocked organization of protein subunits. These studies provide insight into both the assembly and the activity of this complex and suggest a mechanism to enforce fidelity of target binding.

PubMed: 25123481
DOI: 10.1126/science.1256996

実験手法

X-RAY DIFFRACTION (3.0303 Å)