4QXA

Crystal structure of the Rab9A-RUTBC2 RBD complex

4QXA の概要

• エントリーDOI: 10.2210/pdb4qxa/pdb
• 分子名称: Ras-related protein Rab-9A, Small G protein signaling modulator 1, GUANOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: ph domain, rab9a, rutbc2, rab binding domain, rab9-effector complex, protein transport-protein binding complex, protein transport/protein binding
• 由来する生物種: Mus musculus (mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44869.26

構造登録者

Zhang, Z.,Wang, S.,Ding, J. (登録日: 2014-07-19, 公開日: 2014-09-10, 最終更新日: 2023-11-08)

主引用文献

Zhang, Z.,Wang, S.,Shen, T.,Chen, J.,Ding, J.
Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2.
Structure, 22:1408-1420, 2014

PubMed Abstract: Rab9 plays a vital role in regulating the transport of mannose 6-phosphate receptors from late endosomes to the trans-Golgi network through interactions with various effectors. Here, we report the crystal structure of GTP-bound Rab9A in complex with the Rab-binding domain (RBD) of the effector RUTBC2. RUTBC2 RBD assumes a pleckstrin homology domain fold that uses a binding site consisting of mainly β1 and the η1 insertion to interact with the switch and interswitch regions of Rab9A. The C-terminal hypervariable region of Rab9A is disordered and thus not required for RUTBC2 binding. The conformational plasticity of the switch and interswitch regions of Rab9A primarily determines the specificity for RUTBC2. Our biochemical and biological data confirm these findings and further show that Rab9B can bind to RUTBC2 probably in a similar manner as Rab9A. These results together reveal the molecular basis for the binding specificity of Rab9A with RUTBC2.

PubMed: 25220469
DOI: 10.1016/j.str.2014.08.005

実験手法

X-RAY DIFFRACTION (2.3 Å)