4Q75

Crystal structure of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana

Summary for 4Q75

• Entry DOI: 10.2210/pdb4q75/pdb
• Related: 4Q76
• Descriptor: Cysteine desulfurase 2, chloroplastic (2 entities in total)
• Functional Keywords: cysteine desulfurase, atsufe1, chloroplast, transferase
• Biological source: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• Cellular location: Plastid, chloroplast: Q93WX6
• Total number of polymer chains: 2
• Total formula weight: 94418.59

Authors

Roret, T.,Didierjean, C. (deposition date: 2014-04-24, release date: 2014-09-10, Last modification date: 2023-12-06)

Primary citation

Roret, T.,Pegeot, H.,Couturier, J.,Mulliert, G.,Rouhier, N.,Didierjean, C.
X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana.
Acta Crystallogr F Struct Biol Commun, 70:1180-1185, 2014

PubMed Abstract: The chloroplastic Arabidopsis thaliana Nfs2 (AtNfs2) is a group II pyridoxal 5'-phosphate-dependent cysteine desulfurase that is involved in the initial steps of iron-sulfur cluster biogenesis. The group II cysteine desulfurases require the presence of sulfurtransferases such as SufE proteins for optimal activity. Compared with group I cysteine desulfurases, proteins of this group contains a smaller extended lobe harbouring the catalytic cysteine and have a β-hairpin constraining the active site. Here, two crystal structures of AtNfs2 are reported: a wild-type form with the catalytic cysteine in a persulfide-intermediate state and a C384S variant mimicking the resting state of the enzyme. In both structures the well conserved Lys241 covalently binds pyridoxal 5'-phosphate, forming an internal aldimine. Based on available homologous bacterial complexes, a model of a complex between AtNfs2 and the SufE domain of its biological partner AtSufE1 is proposed, revealing the nature of the binding sites.

PubMed: 25195888
DOI: 10.1107/S2053230X14017026

Experimental method

X-RAY DIFFRACTION (1.709 Å)