4Q24

Crystal structure of Cyclo(L-leucyl-L-phenylalanyl) synthase

4Q24 の概要

• エントリーDOI: 10.2210/pdb4q24/pdb
• 関連するPDBエントリー: 3OQV
• 分子名称: Cyclo(L-leucyl-L-phenylalanyl) synthase, PHENYLMETHYL N-[(2S)-4-CHLORO-3-OXO-1-PHENYL-BUTAN-2-YL]CARBAMATE (3 entities in total)
• 機能のキーワード: rossmann fold, cyclodipeptide synthase, aminoacyl-trna, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• 由来する生物種: Streptomyces noursei
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28233.24

構造登録者

Moutiez, M.,Schmitt, E.,Seguin, J.,Thai, R.,Favry, E.,Mechulam, Y.,Gondry, M. (登録日: 2014-04-07, 公開日: 2014-10-08, 最終更新日: 2024-12-25)

主引用文献

Moutiez, M.,Schmitt, E.,Seguin, J.,Thai, R.,Favry, E.,Belin, P.,Mechulam, Y.,Gondry, M.
Unravelling the mechanism of non-ribosomal peptide synthesis by cyclodipeptide synthases.
Nat Commun, 5:5141-5141, 2014

PubMed Abstract: Cyclodipeptide synthases form cyclodipeptides from two aminoacyl transfer RNAs. They use a ping-pong mechanism that begins with transfer of the aminoacyl moiety of the first aminoacyl tRNA onto a conserved serine, yielding an aminoacyl enzyme. Combining X-ray crystallography, site-directed mutagenesis and affinity labelling of the cyclodipeptide synthase AlbC, we demonstrate that the covalent intermediate reacts with the aminoacyl moiety of the second aminoacyl tRNA, forming a dipeptidyl enzyme, and identify the aminoacyl-binding sites of the aminoacyl tRNAs.

PubMed: 25284085
DOI: 10.1038/ncomms6141

実験手法

X-RAY DIFFRACTION (2.9 Å)