4PZ7
PCE1 guanylyltransferase
Summary for 4PZ7
| Entry DOI | 10.2210/pdb4pz7/pdb |
| Related | 4PZ6 4PZ8 |
| Descriptor | mRNA-capping enzyme subunit alpha, SULFATE ION, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | nucleotidyl transferase, rna capping enzyme, rna polymerase ii, spt5, guanylation, nuclear, transferase |
| Biological source | Schizosaccharomyces pombe (Fission yeast) |
| Cellular location | Nucleus: P40997 |
| Total number of polymer chains | 2 |
| Total formula weight | 96858.00 |
| Authors | Doamekpor, S.K.,Lima, C.D. (deposition date: 2014-03-28, release date: 2014-06-25, Last modification date: 2023-09-20) |
| Primary citation | Doamekpor, S.K.,Sanchez, A.M.,Schwer, B.,Shuman, S.,Lima, C.D. How an mRNA capping enzyme reads distinct RNA polymerase II and Spt5 CTD phosphorylation codes. Genes Dev., 28:1323-1336, 2014 Cited by PubMed Abstract: Interactions between RNA guanylyltransferase (GTase) and the C-terminal domain (CTD) repeats of RNA polymerase II (Pol2) and elongation factor Spt5 are thought to orchestrate cotranscriptional capping of nascent mRNAs. The crystal structure of a fission yeast GTase•Pol2 CTD complex reveals a unique docking site on the nucleotidyl transferase domain for an 8-amino-acid Pol2 CTD segment, S5PPSYSPTS5P, bracketed by two Ser5-PO4 marks. Analysis of GTase mutations that disrupt the Pol2 CTD interface shows that at least one of the two Ser5-PO4-binding sites is required for cell viability and that each site is important for cell growth at 37°C. Fission yeast GTase binds the Spt5 CTD at a separate docking site in the OB-fold domain that captures the Trp4 residue of the Spt5 nonapeptide repeat T(1)PAW(4)NSGSK. A disruptive mutation in the Spt5 CTD-binding site of GTase is synthetically lethal with mutations in the Pol2 CTD-binding site, signifying that the Spt5 and Pol2 CTDs cooperate to recruit capping enzyme in vivo. CTD phosphorylation has opposite effects on the interaction of GTase with Pol2 (Ser5-PO4 is required for binding) versus Spt5 (Thr1-PO4 inhibits binding). We propose that the state of Thr1 phosphorylation comprises a binary "Spt5 CTD code" that is read by capping enzyme independent of and parallel to its response to the state of the Pol2 CTD. PubMed: 24939935DOI: 10.1101/gad.242768.114 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.109 Å) |
Structure validation
Download full validation report
