4PXL

Structure of Zm ALDH2-3 (RF2C) in complex with NAD

4PXL の概要

• エントリーDOI: 10.2210/pdb4pxl/pdb
• 関連するPDBエントリー: 3IWJ 4PXN 4PZ2
• 分子名称: Cytosolic aldehyde dehydrogenase RF2C, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, 1,2-ETHANEDIOL, ... (8 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Zea mays (maize); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 114076.73

構造登録者

Morera, S.,Vigouroux, A.,Kopecny, D. (登録日: 2014-03-24, 公開日: 2015-03-18, 最終更新日: 2024-11-27)

主引用文献

Koncitikova, R.,Vigouroux, A.,Kopecna, M.,Andree, T.,Bartos, J.,Sebela, M.,Morera, S.,Kopecny, D.
Role and structural characterization of plant aldehyde dehydrogenases from family 2 and family 7.
Biochem.J., 468:109-123, 2015

PubMed Abstract: Aldehyde dehydrogenases (ALDHs) are responsible for oxidation of biogenic aldehyde intermediates as well as for cell detoxification of aldehydes generated during lipid peroxidation. So far, 13 ALDH families have been described in plants. In the present study, we provide a detailed biochemical characterization of plant ALDH2 and ALDH7 families by analysing maize and pea ALDH7 (ZmALDH7 and PsALDH7) and four maize cytosolic ALDH(cALDH)2 isoforms RF2C, RF2D, RF2E and RF2F [the first maize ALDH2 was discovered as a fertility restorer (RF2A)]. We report the crystal structures of ZmALDH7, RF2C and RF2F at high resolution. The ZmALDH7 structure shows that the three conserved residues Glu(120), Arg(300) and Thr(302) in the ALDH7 family are located in the substrate-binding site and are specific to this family. Our kinetic analysis demonstrates that α-aminoadipic semialdehyde, a lysine catabolism intermediate, is the preferred substrate for plant ALDH7. In contrast, aromatic aldehydes including benzaldehyde, anisaldehyde, cinnamaldehyde, coniferaldehyde and sinapaldehyde are the best substrates for cALDH2. In line with these results, the crystal structures of RF2C and RF2F reveal that their substrate-binding sites are similar and are formed by an aromatic cluster mainly composed of phenylalanine residues and several nonpolar residues. Gene expression studies indicate that the RF2C gene, which is strongly expressed in all organs, appears essential, suggesting that the crucial role of the enzyme would certainly be linked to the cell wall formation using aldehydes from phenylpropanoid pathway as substrates. Finally, plant ALDH7 may significantly contribute to osmoprotection because it oxidizes several aminoaldehydes leading to products known as osmolytes.

PubMed: 25734422
DOI: 10.1042/BJ20150009

実験手法

X-RAY DIFFRACTION (2.25 Å)