4PW9

Crystal structure of the electron-transfer complex formed between a sulfite dehydrogenase and a c-type cytochrome from Sinorhizobium meliloti

4PW9 の概要

• エントリーDOI: 10.2210/pdb4pw9/pdb
• 関連するPDBエントリー: 4PW3 4PWA
• 分子名称: Putative sulfite oxidase, Putative cytochrome C, (MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM, ... (5 entities in total)
• 機能のキーワード: sulfite oxidase, sulfite dehydrogenase, molybdopterin, oxidoreductase, electron transport, electron transfer, electron-transfer complex, c-type cytochrome, heme, oxidoreductase-electron transport complex, oxidoreductase/electron transport
• 由来する生物種: Sinorhizobium meliloti (Ensifer meliloti); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52080.14

構造登録者

McGrath, A.P.,Maher, M.J. (登録日: 2014-03-19, 公開日: 2015-06-03, 最終更新日: 2024-11-06)

主引用文献

McGrath, A.P.,Laming, E.L.,Casas Garcia, G.P.,Kvansakul, M.,Guss, J.M.,Trewhella, J.,Calmes, B.,Bernhardt, P.V.,Hanson, G.R.,Kappler, U.,Maher, M.J.
Structural basis of interprotein electron transfer in bacterial sulfite oxidation.
Elife, 4:e09066-e09066, 2015

PubMed Abstract: Interprotein electron transfer underpins the essential processes of life and relies on the formation of specific, yet transient protein-protein interactions. In biological systems, the detoxification of sulfite is catalyzed by the sulfite-oxidizing enzymes (SOEs), which interact with an electron acceptor for catalytic turnover. Here, we report the structural and functional analyses of the SOE SorT from Sinorhizobium meliloti and its cognate electron acceptor SorU. Kinetic and thermodynamic analyses of the SorT/SorU interaction show the complex is dynamic in solution, and that the proteins interact with Kd = 13.5 ± 0.8 μM. The crystal structures of the oxidized SorT and SorU, both in isolation and in complex, reveal the interface to be remarkably electrostatic, with an unusually large number of direct hydrogen bonding interactions. The assembly of the complex is accompanied by an adjustment in the structure of SorU, and conformational sampling provides a mechanism for dissociation of the SorT/SorU assembly.

PubMed: 26687009
DOI: 10.7554/eLife.09066

実験手法

X-RAY DIFFRACTION (2.49 Å)