4PSM

Crystal structure of pfuThermo-DBP-RP1 (crystal form II)

4PSM の概要

• エントリーDOI: 10.2210/pdb4psm/pdb
• 関連するPDBエントリー: 4PSL 4PSN 4PSO
• 分子名称: ssDNA binding protein, SULFATE ION (3 entities in total)
• 機能のキーワード: ssdna binding protein, dna binding protein
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 70529.54

構造登録者

Gahlei, H.,von Moeller, H.,Eppers, D.,Loll, B.,Wahl, M.C. (登録日: 2014-03-07, 公開日: 2014-04-30, 最終更新日: 2024-11-06)

主引用文献

Ghalei, H.,Moeller, H.v.,Eppers, D.,Sohmen, D.,Wilson, D.N.,Loll, B.,Wahl, M.C.
Entrapment of DNA in an intersubunit tunnel system of a single-stranded DNA-binding protein.
Nucleic Acids Res., 42:6698-6708, 2014

PubMed Abstract: Instead of a classical single-stranded deoxyribonuleic acid (DNA)-binding protein (SSB), some hyperthermophilic crenarchaea harbor a non-canonical SSB termed ThermoDBP. Two related but poorly characterized groups of proteins, which share the ThermoDBP N-terminal DNA-binding domain, have a broader phylogenetic distribution and co-exist with ThermoDBPs and/or other SSBs. We have investigated the nucleic acid binding properties and crystal structures of representatives of these groups of ThermoDBP-related proteins (ThermoDBP-RPs) 1 and 2. ThermoDBP-RP 1 and 2 oligomerize by different mechanisms and only ThermoDBP-RP2 exhibits strong single-stranded DNA affinity in vitro. A crystal structure of ThermoDBP-RP2 in complex with DNA reveals how the NTD common to ThermoDBPs and ThermoDBP-RPs can contact the nucleic acid in a manner that allows a symmetric homotetrameric protein complex to bind single-stranded DNA molecules asymmetrically. While single-stranded DNA wraps around the surface or binds along channels of previously investigated SSBs, it traverses an internal, intersubunit tunnel system of a ThermoDBP-RP2 tetramer. Our results indicate that some archaea have acquired special SSBs for genome maintenance in particularly challenging environments.

PubMed: 24744237
DOI: 10.1093/nar/gku259

実験手法

X-RAY DIFFRACTION (2.43 Å)