4PSI

PIH1D1/phospho-Tel2 complex

Summary for 4PSI

• Entry DOI: 10.2210/pdb4psi/pdb
• Related: 4PSF
• Descriptor: PIH1 domain-containing protein 1, Telomere length regulation protein TEL2 homolog (3 entities in total)
• Functional Keywords: alpha, beta, phospho-binding, tel2, phsophorylation, protein binding
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm: Q9Y4R8
• Total number of polymer chains: 4
• Total formula weight: 34182.25

Authors

Smerdon, S.J.,Boulton, S.J.,Stach, L.,Flower, T.G.,Horejsi, Z. (deposition date: 2014-03-07, release date: 2014-04-23, Last modification date: 2024-11-06)

Primary citation

Horejsi, Z.,Stach, L.,Flower, T.G.,Joshi, D.,Flynn, H.,Skehel, J.M.,O'Reilly, N.J.,Ogrodowicz, R.W.,Smerdon, S.J.,Boulton, S.J.
Phosphorylation-Dependent PIH1D1 Interactions Define Substrate Specificity of the R2TP Cochaperone Complex.
Cell Rep, 7:19-26, 2014

PubMed Abstract: The R2TP cochaperone complex plays a critical role in the assembly of multisubunit machines, including small nucleolar ribonucleoproteins (snoRNPs), RNA polymerase II, and the mTORC1 and SMG1 kinase complexes, but the molecular basis of substrate recognition remains unclear. Here, we describe a phosphopeptide binding domain (PIH-N) in the PIH1D1 subunit of the R2TP complex that preferentially binds to highly acidic phosphorylated proteins. A cocrystal structure of a PIH-N domain/TEL2 phosphopeptide complex reveals a highly specific phosphopeptide recognition mechanism in which Lys57 and 64 in PIH1D1, along with a conserved DpSDD phosphopeptide motif within TEL2, are essential and sufficient for binding. Proteomic analysis of PIH1D1 interactors identified R2TP complex substrates that are recruited by the PIH-N domain in a sequence-specific and phosphorylation-dependent manner suggestive of a common mechanism of substrate recognition. We propose that protein complexes assembled by the R2TP complex are defined by phosphorylation of a specific motif and recognition by the PIH1D1 subunit.

PubMed: 24656813
DOI: 10.1016/j.celrep.2014.03.013

Experimental method

X-RAY DIFFRACTION (2.45 Å)