4PSB

Crystal Structure of Phytohormone Binding Protein from Vigna radiata in complex with gibberellic acid (GA3)

4PSB の概要

• エントリーDOI: 10.2210/pdb4psb/pdb
• 関連するPDBエントリー: 2flh 2qim 3us7 4jhg
• 分子名称: Cytokinin-specific binding protein, GIBBERELLIN A3 (3 entities in total)
• 機能のキーワード: cytokinin specific binding protein (csbp), pr-10 fold, gibberellin, cytokinin, plant hormone binding, plant protein
• 由来する生物種: Vigna radiata (mung bean)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17959.23

構造登録者

Ruszkowski, M.,Sikorski, M.,Jaskolski, M. (登録日: 2014-03-07, 公開日: 2014-07-23, 最終更新日: 2023-09-20)

主引用文献

Ruszkowski, M.,Sliwiak, J.,Ciesielska, A.,Barciszewski, J.,Sikorski, M.,Jaskolski, M.
Specific binding of gibberellic acid by Cytokinin-Specific Binding Proteins: a new aspect of plant hormone-binding proteins with the PR-10 fold.
Acta Crystallogr.,Sect.D, 70:2032-2041, 2014

PubMed Abstract: Pathogenesis-related proteins of class 10 (PR-10) are a family of plant proteins with the same fold characterized by a large hydrophobic cavity that allows them to bind various ligands, such as phytohormones. A subfamily with only ~20% sequence identity but with a conserved canonical PR-10 fold have previously been recognized as Cytokinin-Specific Binding Proteins (CSBPs), although structurally the binding mode of trans-zeatin (a cytokinin phytohormone) was found to be quite diversified. Here, it is shown that two CSBP orthologues from Medicago truncatula and Vigna radiata bind gibberellic acid (GA3), which is an entirely different phytohormone, in a conserved and highly specific manner. In both cases a single GA3 molecule is found in the internal cavity of the protein. The structural data derived from high-resolution crystal structures are corroborated by isothermal titration calorimetry (ITC), which reveals a much stronger interaction with GA3 than with trans-zeatin and pH dependence of the binding profile. As a conclusion, it is postulated that the CSBP subfamily of plant PR-10 proteins should be more properly linked with general phytohormone-binding properties and termed phytohormone-binding proteins (PhBP).

PubMed: 25004979
DOI: 10.1107/S1399004714010578

実験手法

X-RAY DIFFRACTION (1.42 Å)