4PNF

Glutathione S-Transferase from Drosophila melanogaster - isozyme E6

4PNF の概要

• エントリーDOI: 10.2210/pdb4pnf/pdb
• 分子名称: RE21095p, GLUTATHIONE (3 entities in total)
• 機能のキーワード: glutathione s-transferase, isozyme, glutathione complex, transferase
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 208380.58

構造登録者

Scian, M.,Le Trong, I.,Mannervik, B.,Atkins, W.M.,Stenkamp, R.E. (登録日: 2014-05-23, 公開日: 2015-05-27, 最終更新日: 2023-12-27)

主引用文献

Scian, M.,Le Trong, I.,Mazari, A.M.,Mannervik, B.,Atkins, W.M.,Stenkamp, R.E.
Comparison of epsilon- and delta-class glutathione S-transferases: the crystal structures of the glutathione S-transferases DmGSTE6 and DmGSTE7 from Drosophila melanogaster.
Acta Crystallogr.,Sect.D, 71:2089-2098, 2015

PubMed Abstract: Cytosolic glutathione transferases (GSTs) comprise a large family of enzymes with canonical structures that diverge functionally and structurally among mammals, invertebrates and plants. Whereas mammalian GSTs have been characterized extensively with regard to their structure and function, invertebrate GSTs remain relatively unstudied. The invertebrate GSTs do, however, represent potentially important drug targets for infectious diseases and agricultural applications. In addition, it is essential to fully understand the structure and function of invertebrate GSTs, which play important roles in basic biological processes. Invertebrates harbor delta- and epsilon-class GSTs, which are not found in other organisms. Drosophila melanogaster GSTs (DmGSTs) are likely to contribute to detoxication or antioxidative stress during development, but they have not been fully characterized. Here, the structures of two epsilon-class GSTs from Drosophila, DmGSTE6 and DmGSTE7, are reported at 2.1 and 1.5 Å resolution, respectively, and are compared with other GSTs to identify structural features that might correlate with their biological functions. The structures of DmGSTE6 and DmGSTE7 are remarkably similar; the structures do not reveal obvious sources of the minor functional differences that have been observed. The main structural difference between the epsilon- and delta-class GSTs is the longer helix (A8) at the C-termini of the epsilon-class enzymes.

PubMed: 26457432
DOI: 10.1107/S1399004715013929

実験手法

X-RAY DIFFRACTION (2.11 Å)