4PMH

The structure of rice weevil pectin methyl esterase

4PMH の概要

• エントリーDOI: 10.2210/pdb4pmh/pdb
• 分子名称: Pectinesterase (2 entities in total)
• 機能のキーワード: beta-barrel, pectin methyl esterase, hydrolase
• 由来する生物種: Sitophilus oryzae (Rice weevil)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39044.50

構造登録者

Stenkamp, R.E.,Teller, D.C.,Behnke, C.A.,Reeck, G.R. (登録日: 2014-05-21, 公開日: 2014-11-12, 最終更新日: 2024-10-23)

主引用文献

Teller, D.C.,Behnke, C.A.,Pappan, K.,Shen, Z.,Reese, J.C.,Reeck, G.R.,Stenkamp, R.E.
The structure of rice weevil pectin methylesterase.
Acta Crystallogr.,Sect.F, 70:1480-1484, 2014

PubMed Abstract: Rice weevils (Sitophilus oryzae) use a pectin methylesterase (EC 3.1.1.11), along with other enzymes, to digest cell walls in cereal grains. The enzyme is a right-handed β-helix protein, but is circularly permuted relative to plant and bacterial pectin methylesterases, as shown by the crystal structure determination reported here. This is the first structure of an animal pectin methylesterase. Diffraction data were collected to 1.8 Å resolution some time ago for this crystal form, but structure solution required the use of molecular-replacement techniques that have been developed and similar structures that have been deposited in the last 15 years. Comparison of the structure of the rice weevil pectin methylesterase with that from Dickeya dandantii (formerly Erwinia chrysanthemi) indicates that the reaction mechanisms are the same for the insect, plant and bacterial pectin methylesterases. The similarity of the structure of the rice weevil enzyme to the Escherichia coli lipoprotein YbhC suggests that the evolutionary origin of the rice weevil enzyme was a bacterial lipoprotein, the gene for which was transferred to a primitive ancestor of modern weevils and other Curculionidae. Structural comparison of the rice weevil pectin methylesterase with plant and bacterial enzymes demonstrates that the rice weevil protein is circularly permuted relative to the plant and bacterial molecules.

PubMed: 25372813
DOI: 10.1107/S2053230X14020433

実験手法

X-RAY DIFFRACTION (1.79 Å)