4PLO

Crystal Structure of chicken Netrin-1 (LN-LE3) in complex with mouse DCC (FN4-5)

4PLO の概要

• エントリーDOI: 10.2210/pdb4plo/pdb
• 関連するPDBエントリー: 4PLM 4PLN
• 分子名称: Netrin receptor DCC, Netrin-1, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: elongated, complex, protein binding
• 由来する生物種: Gallus gallus (Chicken); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72496.12

構造登録者

Xu, K.,Nikolov, D.B. (登録日: 2014-05-18, 公開日: 2014-06-18, 最終更新日: 2024-11-20)

主引用文献

Xu, K.,Wu, Z.,Renier, N.,Antipenko, A.,Tzvetkova-Robev, D.,Xu, Y.,Minchenko, M.,Nardi-Dei, V.,Rajashankar, K.R.,Himanen, J.,Tessier-Lavigne, M.,Nikolov, D.B.
Neural migration. Structures of netrin-1 bound to two receptors provide insight into its axon guidance mechanism.
Science, 344:1275-1279, 2014

PubMed Abstract: Netrins are secreted proteins that regulate axon guidance and neuronal migration. Deleted in colorectal cancer (DCC) is a well-established netrin-1 receptor mediating attractive responses. We provide evidence that its close relative neogenin is also a functional netrin-1 receptor that acts with DCC to mediate guidance in vivo. We determined the structures of a functional netrin-1 region, alone and in complexes with neogenin or DCC. Netrin-1 has a rigid elongated structure containing two receptor-binding sites at opposite ends through which it brings together receptor molecules. The ligand/receptor complexes reveal two distinct architectures: a 2:2 heterotetramer and a continuous ligand/receptor assembly. The differences result from different lengths of the linker connecting receptor domains fibronectin type III domain 4 (FN4) and FN5, which differs among DCC and neogenin splice variants, providing a basis for diverse signaling outcomes.

PubMed: 24876346
DOI: 10.1126/science.1255149

実験手法

X-RAY DIFFRACTION (2.901 Å)