4PHY

Functional conservation despite structural divergence in ligand-responsive RNA switches

4PHY の概要

• エントリーDOI: 10.2210/pdb4phy/pdb
• 分子名称: RNA (26-MER), RNA (5'-R(*GP*CP*AP*GP*GP*AP*AP*CP*CP*GP*AP*GP*AP*GP*GP*CP*AP*CP*GP*C)-3'), MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: viral genome, internal ribosome entry site, translation, rna
• 由来する生物種: Seneca valley virus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14896.62

構造登録者

Boerneke, M.A.,Dibrov, S.M.,Hermann, T.H. (登録日: 2014-05-07, 公開日: 2015-02-18, 最終更新日: 2023-12-27)

主引用文献

Boerneke, M.A.,Dibrov, S.M.,Gu, J.,Wyles, D.L.,Hermann, T.
Functional conservation despite structural divergence in ligand-responsive RNA switches.
Proc.Natl.Acad.Sci.USA, 111:15952-15957, 2014

PubMed Abstract: An internal ribosome entry site (IRES) initiates protein synthesis in RNA viruses, including the hepatitis C virus (HCV). We have discovered ligand-responsive conformational switches in viral IRES elements. Modular RNA motifs of greatly distinct sequence and local secondary structure have been found to serve as functionally conserved switches involved in viral IRES-driven translation and may be captured by identical cognate ligands. The RNA motifs described here constitute a new paradigm for ligand-captured switches that differ from metabolite-sensing riboswitches with regard to their small size, as well as the intrinsic stability and structural definition of the constitutive conformational states. These viral RNA modules represent the simplest form of ligand-responsive mechanical switches in nucleic acids.

PubMed: 25349403
DOI: 10.1073/pnas.1414678111

実験手法

X-RAY DIFFRACTION (3.1 Å)