4PGU

Crystal structure of YetJ from Bacillus Subtilis at pH 7 by soaking

4PGU の概要

• エントリーDOI: 10.2210/pdb4pgu/pdb
• 関連するPDBエントリー: 4PGR 4PGS 4PGV 4PGW
• 分子名称: Uncharacterized protein YetJ (1 entity in total)
• 機能のキーワード: membrane protein, 7-tms, triple-helix sandwich, di-aspartyl ph sensor, calcium leak, conformational equilibrium, structural genomics, psi-biology, new york consortium on membrane protein structure, nycomps, membrance protein
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24116.81

構造登録者

Liu, Q.,Chang, Y.,Hendrickson, W.A.,New York Consortium on Membrane Protein Structure (NYCOMPS) (登録日: 2014-05-02, 公開日: 2014-06-04, 最終更新日: 2023-12-27)

主引用文献

Chang, Y.,Bruni, R.,Kloss, B.,Assur, Z.,Kloppmann, E.,Rost, B.,Hendrickson, W.A.,Liu, Q.
Structural basis for a pH-sensitive calcium leak across membranes.
Science, 344:1131-1135, 2014

PubMed Abstract: Calcium homeostasis balances passive calcium leak and active calcium uptake. Human Bax inhibitor-1 (hBI-1) is an antiapoptotic protein that mediates a calcium leak and is representative of a highly conserved and widely distributed family, the transmembrane Bax inhibitor motif (TMBIM) proteins. Here, we present crystal structures of a bacterial homolog and characterize its calcium leak activity. The structure has a seven-transmembrane-helix fold that features two triple-helix sandwiches wrapped around a central C-terminal helix. Structures obtained in closed and open conformations are reversibly interconvertible by change of pH. A hydrogen-bonded, pKa (where Ka is the acid dissociation constant)-perturbed pair of conserved aspartate residues explains the pH dependence of this transition, and biochemical studies show that pH regulates calcium influx in proteoliposomes. Homology models for hBI-1 provide insights into TMBIM-mediated calcium leak and cytoprotective activity.

PubMed: 24904158
DOI: 10.1126/science.1252043

実験手法

X-RAY DIFFRACTION (3.401 Å)