4PGC

MHC Class I in complex with modified Sendai virus nucleoprotein peptide FAPGN(3,5-diiodotyrosine)PAL

4PGC の概要

• エントリーDOI: 10.2210/pdb4pgc/pdb
• 関連するPDBエントリー: 4PG9 4PGB 4PGD 4PGE
• 分子名称: H-2 class I histocompatibility antigen, K-B alpha chain, Beta-2-microglobulin, Sendai virus nucleoprotein, ... (6 entities in total)
• 機能のキーワード: immune system, immunoglobulin domain, immune response, immune system-peptide complex, immune system/peptide
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 97753.85

構造登録者

Celie, P.,Joosten, R.P.,Perrakis, A.,Neefjes, J. (登録日: 2014-05-01, 公開日: 2015-01-07, 最終更新日: 2024-11-06)

主引用文献

Garstka, M.A.,Fish, A.,Celie, P.H.,Joosten, R.P.,Janssen, G.M.,Berlin, I.,Hoppes, R.,Stadnik, M.,Janssen, L.,Ovaa, H.,van Veelen, P.A.,Perrakis, A.,Neefjes, J.
The first step of peptide selection in antigen presentation by MHC class I molecules.
Proc.Natl.Acad.Sci.USA, 112:1505-1510, 2015

PubMed Abstract: MHC class I molecules present a variable but limited repertoire of antigenic peptides for T-cell recognition. Understanding how peptide selection is achieved requires mechanistic insights into the interactions between the MHC I and candidate peptides. We find that, at first encounter, MHC I H-2K(b) considers a wide range of peptides, including those with expanded N termini and unfitting anchor residues. Discrimination occurs in the second step, when noncanonical peptides dissociate with faster exchange rates. This second step exhibits remarkable temperature sensitivity, as illustrated by numerous noncanonical peptides presented by H-2K(b) in cells cultured at 26 °C relative to 37 °C. Crystallographic analyses of H-2K(b)-peptide complexes suggest that a conformational adaptation of H-2K(b) drives the decisive step in peptide selection. We propose that MHC class I molecules consider initially a large peptide pool, subsequently refined by a temperature-sensitive induced-fit mechanism to retain the canonical peptide repertoire.

PubMed: 25605945
DOI: 10.1073/pnas.1416543112

実験手法

X-RAY DIFFRACTION (2.3 Å)