4PFE

Crystal structure of vsfGFP-0

4PFE の概要

• エントリーDOI: 10.2210/pdb4pfe/pdb
• 分子名称: Green fluorescent protein (2 entities in total)
• 機能のキーワード: beta barrel, fusion protein, homodimer, immune system, fluorescent protein
• 由来する生物種: Aequorea victoria (Jellyfish); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 77122.22

構造登録者

Jauch, R.,Chen, S.L. (登録日: 2014-04-29, 公開日: 2015-06-24, 最終更新日: 2024-11-06)

主引用文献

Eshaghi, M.,Sun, G.,Gruter, A.,Lim, C.L.,Chee, Y.C.,Jung, G.,Jauch, R.,Wohland, T.,Chen, S.L.
Rational Structure-Based Design of Bright GFP-Based Complexes with Tunable Dimerization.
Angew.Chem.Int.Ed.Engl., 54:13952-13956, 2015

PubMed Abstract: Fluorescent proteins are transformative tools; thus, any brightness increase is a welcome improvement. We invented the "vGFP strategy" based on structural analysis of GFP bound to a single-domain antibody, predicting tunable dimerization, enhanced brightness (ca. 50%), and improved pH resistance. We verified all of these predictions using biochemistry, crystallography, and single-molecule studies. We applied the vsfGFP proteins in three diverse scenarios: single-step immunofluorescence in vitro (3× brighter due to dimerization); expression in bacteria and human cells in vivo (1.5× brighter); and protein fusions showing better pH resistance in human cells in vivo. The vGFP strategy thus allows upgrading of existing applications, is applicable to other fluorescent proteins, and suggests a method for tuning dimerization of arbitrary proteins and optimizing protein properties in general.

PubMed: 26447926
DOI: 10.1002/anie.201506686

実験手法

X-RAY DIFFRACTION (2.603 Å)