4PD3

Crystal Structure of Rigor-Like Human Nonmuscle Myosin-2B

4PD3 の概要

• エントリーDOI: 10.2210/pdb4pd3/pdb
• 分子名称: Nonmuscle myosin heavy chain B, Alpha-actinin A Chimera Protein (2 entities in total)
• 機能のキーワード: myosin, nm-2b, nonmuscle myosin-2b, rigor-like, nucleotide free, motor protein, contractile protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 237434.09

構造登録者

Munnich, S.,Pathan-Chhatbar, S.,Manstein, D.J. (登録日: 2014-04-17, 公開日: 2014-11-12, 最終更新日: 2023-12-27)

主引用文献

Munnich, S.,Pathan-Chhatbar, S.,Manstein, D.J.
Crystal structure of the rigor-like human non-muscle myosin-2 motor domain.
Febs Lett., 588:4754-4760, 2014

PubMed Abstract: We determined the crystal structure of the motor domain of human non-muscle myosin 2B (NM-2B) in a nucleotide-free state and at a resolution of 2.8 Å. The structure shows the motor domain with an open active site and the large cleft that divides the 50 kDa domain in a closed state. Compared to other rigor-like myosin motor domain structures, our structure shows subtle but significant conformational changes in regions important for actin binding and mechanochemical coupling. Moreover, our crystal structure helps to rationalize the impact of myosin, heavy chain 9 (MYH9)-related disease mutations Arg709Cys and Arg709His on the kinetic and functional properties of NM-2B and of the closely related non-muscle myosin 2A (NM-2A).

PubMed: 25451231
DOI: 10.1016/j.febslet.2014.11.007

実験手法

X-RAY DIFFRACTION (2.84 Å)