4PD1

Structure of gephyrin E domain with Glycine-beta receptor peptide

Summary for 4PD1

• Entry DOI: 10.2210/pdb4pd1/pdb
• Related: 4PD0
• Descriptor: Gephyrin, Glycine receptor subunit beta, GLYCEROL, ... (5 entities in total)
• Functional Keywords: scaffolding protein, neurotransmitter receptor anchoring protein, molybdenum cofactor biosynthesis, structural protein-signaling protein complex, structural protein-transport protein complex, structural protein/transport protein
• Biological source: Rattus norvegicus (Rat); More
• Total number of polymer chains: 2
• Total formula weight: 48131.04

Authors

Kasaragod, V.B.,Maric, H.M.,Schindelin, H. (deposition date: 2014-04-17, release date: 2014-08-27, Last modification date: 2023-09-27)

Primary citation

Maric, H.M.,Kasaragod, V.B.,Schindelin, H.
Modulation of gephyrin-glycine receptor affinity by multivalency.
Acs Chem.Biol., 9:2554-2562, 2014

PubMed Abstract: Gephyrin is a major determinant for the accumulation and anchoring of glycine receptors (GlyRs) and the majority of γ-aminobutyric acid type A receptors (GABAARs) at postsynaptic sites. Here we explored the interaction of gephyrin with a dimeric form of a GlyR β-subunit receptor-derived peptide. A 2 Å crystal structure of the C-terminal domain of gephyrin (GephE) in complex with a 15-residue peptide derived from the GlyR β-subunit defined the core binding site, which we targeted with the dimeric peptide. Biophysical analyses via differential scanning calorimetry (DSC), thermofluor, and isothermal titration calorimetry (ITC) demonstrated that this dimeric ligand is capable of binding simultaneously to two receptor binding sites and that this multivalency results in a 25-fold enhanced affinity. Our study therefore suggests that the oligomeric state of gephyrin and the number of gephyrin-binding subunits in the pentameric GABAARs and GlyRs together control postsynaptic receptor clustering.

PubMed: 25137389
DOI: 10.1021/cb500303a

Experimental method

X-RAY DIFFRACTION (1.975 Å)