4PCV

The structure of BdcA (YjgI) from E. coli

4PCV の概要

• エントリーDOI: 10.2210/pdb4pcv/pdb
• 分子名称: BdcA (YjgI), TRIETHYLENE GLYCOL, PENTAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: nadp(h) oxidoreductase, oxidoreductase
• 由来する生物種: Escherichia coli P0299438.9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50595.72

構造登録者

Page, R.,Peti, W.,Lord, D. (登録日: 2014-04-16, 公開日: 2015-03-11, 最終更新日: 2023-12-27)

主引用文献

Lord, D.M.,Baran, A.U.,Wood, T.K.,Peti, W.,Page, R.
BdcA, a protein important for Escherichia coli biofilm dispersal, is a short-chain dehydrogenase/reductase that binds specifically to NADPH.
Plos One, 9:e105751-e105751, 2014

PubMed Abstract: The Escherichia coli protein BdcA (previously referred to as YjgI) plays a key role in the dispersal of cells from bacterial biofilms, and its constitutive activation provides an attractive therapeutic target for dismantling these communities. In order to investigate the function of BdcA at a molecular level, we integrated structural and functional studies. Our 2.05 Å structure of BdcA shows that it is a member of the NAD(P)(H)-dependent short-chain dehydrogenase/reductase (SDR) superfamily. Structural comparisons with other members of the SDR family suggested that BdcA binds NADP(H). This was demonstrated experimentally using thermal denaturation studies, which showed that BcdA binds specifically to NADPH. Subsequent ITC experiments further confirmed this result and reported a Kd of 25.9 µM. Thus, BdcA represents the newest member of the limited number of oxidoreductases shown to be involved in quorum sensing and biofilm dispersal.

PubMed: 25244619
DOI: 10.1371/journal.pone.0105751

実験手法

X-RAY DIFFRACTION (2.05 Å)