4P1W

Crystal structure of Atg13(17BR)-Atg17-Atg29-Atg31 complex

4P1W の概要

• エントリーDOI: 10.2210/pdb4p1w/pdb
• 関連するPDBエントリー: 4P1N
• 分子名称: Atg29, Atg31, Atg17, ... (4 entities in total)
• 機能のキーワード: complex, protein transport
• 由来する生物種: Lachancea thermotolerans (Yeast); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 148419.84

構造登録者

Fujioka, Y.,Noda, N.N. (登録日: 2014-02-27, 公開日: 2014-05-07, 最終更新日: 2024-11-13)

主引用文献

Fujioka, Y.,Suzuki, S.W.,Yamamoto, H.,Kondo-Kakuta, C.,Kimura, Y.,Hirano, H.,Akada, R.,Inagaki, F.,Ohsumi, Y.,Noda, N.N.
Structural basis of starvation-induced assembly of the autophagy initiation complex.
Nat.Struct.Mol.Biol., 21:513-521, 2014

PubMed Abstract: Assembly of the preautophagosomal structure (PAS) is essential for autophagy initiation in yeast. Starvation-induced dephosphorylation of Atg13 is required for the formation of the Atg1-Atg13-Atg17-Atg29-Atg31 complex (Atg1 complex), a prerequisite for PAS assembly. However, molecular details underlying these events have not been established. Here we studied the interactions of yeast Atg13 with Atg1 and Atg17 by X-ray crystallography. Atg13 binds tandem microtubule interacting and transport domains in Atg1, using an elongated helix-loop-helix region. Atg13 also binds Atg17, using a short region, thereby bridging Atg1 and Atg17 and leading to Atg1-complex formation. Dephosphorylation of specific serines in Atg13 enhanced its interaction with not only Atg1 but also Atg17. These observations update the autophagy-initiation model as follows: upon starvation, dephosphorylated Atg13 binds both Atg1 and Atg17, and this promotes PAS assembly and autophagy progression.

PubMed: 24793651
DOI: 10.1038/nsmb.2822

実験手法

X-RAY DIFFRACTION (3.2 Å)