4OZU

Crystal Structure of WD40 domain from Toxoplasma gondii coronin

4OZU の概要

• エントリーDOI: 10.2210/pdb4ozu/pdb
• 分子名称: Coronin, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: wd40 repeat, structural protein, actin binding protein, apicomplexan parasite
• 由来する生物種: Toxoplasma gondii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43635.48

構造登録者

Kallio, J.P.,Kursula, I. (登録日: 2014-02-19, 公開日: 2014-08-20, 最終更新日: 2024-10-23)

主引用文献

Salamun, J.,Kallio, J.P.,Daher, W.,Soldati-Favre, D.,Kursula, I.
Structure of Toxoplasma gondii coronin, an actin-binding protein that relocalizes to the posterior pole of invasive parasites and contributes to invasion and egress.
Faseb J., 28:4729-4747, 2014

PubMed Abstract: Coronins are involved in the regulation of actin dynamics in a multifaceted way, participating in cell migration and vesicular trafficking. Apicomplexan parasites, which exhibit an actin-dependent gliding motility that is essential for traversal through tissues, as well as invasion of and egress from host cells, express only a single coronin, whereas higher eukaryotes possess several isoforms. We set out to characterize the 3-D structure, biochemical function, subcellular localization, and genetic ablation of Toxoplasma gondii coronin (TgCOR), to shed light on its biological role. A combination of X-ray crystallography, small-angle scattering of X-rays, and light scattering revealed the atomic structure of the conserved WD40 domain and the dimeric arrangement of the full-length protein. TgCOR binds to F-actin and increases the rate and extent of actin polymerization. In vivo, TgCOR relocalizes transiently to the posterior pole of motile and invading parasites, independent of actin dynamics, but concomitant to microneme secretory organelle discharge. TgCOR contributes to, but is not essential for, invasion and egress. Taken together, our data point toward a role for TgCOR in stabilizing newly formed, short filaments and F-actin cross-linking, as well as functions linked to endocytosis and recycling of membranes.

PubMed: 25114175
DOI: 10.1096/fj.14-252569

実験手法

X-RAY DIFFRACTION (1.65 Å)