4OWT

Structural basis of SOSS1 complex assembly

4OWT の概要

• エントリーDOI: 10.2210/pdb4owt/pdb
• 関連するPDBエントリー: 4OWX
• 分子名称: Integrator complex subunit 3, SOSS complex subunit B1, SOSS complex subunit C, ... (4 entities in total)
• 機能のキーワード: soss1 complex, dna double-strand breaks, homologous recombination, ssdna- binding protein, dna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 87954.32

構造登録者

Ren, W.,Sun, Q.,Tang, X.,Song, H. (登録日: 2014-02-03, 公開日: 2014-04-16, 最終更新日: 2024-10-09)

主引用文献

Ren, W.,Chen, H.,Sun, Q.,Tang, X.,Lim, S.C.,Huang, J.,Song, H.
Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA.
Cell Rep, 6:982-991, 2014

PubMed Abstract: The SOSS1 complex comprising SOSSA, SOSSB1, and SOSSC senses single-stranded DNA (ssDNA) and promotes repair of DNA double-strand breaks (DSBs). But how SOSS1 is assembled and recognizes ssDNA remains elusive. The crystal structure of the N-terminal half of SOSSA (SOSSAN) in complex with SOSSB1 and SOSSC showed that SOSSAN serves as a scaffold to bind both SOSSB1 and SOSSC for assembly of the SOSS1 complex. The structures of SOSSAN/B1 in complex with a 12 nt ssDNA and SOSSAN/B1/C in complex with a 35 nt ssDNA showed that SOSSB1 interacts with both SOSSAN and ssDNA via two distinct surfaces. Recognition of ssDNA with a length of up to nine nucleotides is mediated solely by SOSSB1, whereas neither SOSSC nor SOSSAN are critical for ssDNA binding. These results reveal the structural basis of SOSS1 assembly and provide a framework for further study of the mechanism governing longer ssDNA recognition by the SOSS1 complex during DSB repair.

PubMed: 24630995
DOI: 10.1016/j.celrep.2014.02.020

実験手法

X-RAY DIFFRACTION (2 Å)