4OUD

Engineered tyrosyl-tRNA synthetase with the nonstandard amino acid L-4,4-biphenylalanine

4OUD の概要

• エントリーDOI: 10.2210/pdb4oud/pdb
• 分子名称: Tyrosyl-tRNA synthetase, TYROSINE, ... (4 entities in total)
• 機能のキーワード: complex with l-tyrosine, rossmann fold, ligase, trna
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm : U6N9P2 U6N9P2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 91111.12

構造登録者

Takeuchi, R.,Mandell, D.J.,Lajoie, M.J.,Church, G.M.,Stoddard, B.L. (登録日: 2014-02-16, 公開日: 2015-01-28, 最終更新日: 2023-09-20)

主引用文献

Mandell, D.J.,Lajoie, M.J.,Mee, M.T.,Takeuchi, R.,Kuznetsov, G.,Norville, J.E.,Gregg, C.J.,Stoddard, B.L.,Church, G.M.
Biocontainment of genetically modified organisms by synthetic protein design.
Nature, 518:55-60, 2015

PubMed Abstract: Genetically modified organisms (GMOs) are increasingly deployed at large scales and in open environments. Genetic biocontainment strategies are needed to prevent unintended proliferation of GMOs in natural ecosystems. Existing biocontainment methods are insufficient because they impose evolutionary pressure on the organism to eject the safeguard by spontaneous mutagenesis or horizontal gene transfer, or because they can be circumvented by environmentally available compounds. Here we computationally redesign essential enzymes in the first organism possessing an altered genetic code (Escherichia coli strain C321.ΔA) to confer metabolic dependence on non-standard amino acids for survival. The resulting GMOs cannot metabolically bypass their biocontainment mechanisms using known environmental compounds, and they exhibit unprecedented resistance to evolutionary escape through mutagenesis and horizontal gene transfer. This work provides a foundation for safer GMOs that are isolated from natural ecosystems by a reliance on synthetic metabolites.

PubMed: 25607366
DOI: 10.1038/nature14121

実験手法

X-RAY DIFFRACTION (2.65 Å)