4OUA

Coexistent single-crystal structure of latent and active mushroom tyrosinase (abPPO4) mediated by a hexatungstotellurate(VI)

4OUA の概要

• エントリーDOI: 10.2210/pdb4oua/pdb
• 分子名称: proteolytically activated form of PPO4 Tyrosinase, latent form of PPO4 Tyrosinase, COPPER (I) ION, ... (7 entities in total)
• 機能のキーワード: oxidoreductase, type-3 copper protein, tyrosinase, ppo4, zymogen, tyrosinase maturation, hetero-protein co-crystallization, anderson-evans-type polyoxometalate
• 由来する生物種: Agaricus bisporus var. bisporus H97 (White button mushroom); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 111434.95

構造登録者

St.Mauracher, G.,Molitor, C.,Al-Oweini, R.,Kortz, U.,Rompel, A. (登録日: 2014-02-15, 公開日: 2014-06-25, 最終更新日: 2018-01-31)

主引用文献

Mauracher, S.G.,Molitor, C.,Al-Oweini, R.,Kortz, U.,Rompel, A.
Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal.
Acta Crystallogr.,Sect.D, 70:2301-2315, 2014

PubMed Abstract: Tyrosinases, bifunctional metalloenzymes, catalyze the oxidation of monophenols and o-diphenols to o-quinones, the precursor compounds of the brown-coloured pigment melanin. In eukaryotic organisms, tyrosinases are expressed as latent zymogens that have to be proteolytically cleaved in order to form highly active enzymes. This activation mechanism, known as the tyrosinase maturation process, has scientific and industrial significance with respect to biochemical and technical applications of the enzyme. Here, not only the first crystal structure of the mushroom tyrosinase abPPO4 is presented in its active form (Ser2-Ser383) and in its 21 kDa heavier latent form (Ser2-Thr545), but furthermore the simultaneous presence of both forms within one single-crystal structure is shown. This allows for a simple approach to investigate the transition between these two forms. Isoform abPPO4 was isolated and extensively purified from the natural source (Agaricus bisporus), which contains a total of six polyphenol oxidases (PPOs). The enzyme formed crystals (diffracting to a resolution of 2.76 Å) owing to the employment of the 6-tungstotellurate(VI) salt (Na6[TeW6O24]·22H2O) as a cocrystallization agent. Two of these disc-shaped Anderson-type polyoxoanions [TeW6O24](6-) separate two asymmetric units comprising one crystallographic heterodimer of abPPO4, thus resulting in very interesting crystal packing.

PubMed: 25195745
DOI: 10.1107/S1399004714013777

実験手法

X-RAY DIFFRACTION (2.763 Å)