4OU6

Crystal structure of DnaT84-153-dT10 ssDNA complex form 1

4OU6 の概要

• エントリーDOI: 10.2210/pdb4ou6/pdb
• 関連するPDBエントリー: 4OU7
• 分子名称: Primosomal protein 1, DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') (3 entities in total)
• 機能のキーワード: dna binding, replication-dna complex, replication/dna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 46031.10

構造登録者

Liu, Z.,Chen, P.,Niu, L.,Teng, M.,Li, X. (登録日: 2014-02-15, 公開日: 2014-08-13, 最終更新日: 2024-05-29)

主引用文献

Liu, Z.,Chen, P.,Wang, X.,Cai, G.,Niu, L.,Teng, M.,Li, X.
Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode.
Nucleic Acids Res., 42:9470-9483, 2014

PubMed Abstract: DnaT is a primosomal protein that is required for the stalled replication fork restart in Escherichia coli. As an adapter, DnaT mediates the PriA-PriB-ssDNA ternary complex and the DnaB/C complex. However, the fundamental function of DnaT during PriA-dependent primosome assembly is still a black box. Here, we report the 2.83 Å DnaT(84-153)-dT10 ssDNA complex structure, which reveals a novel three-helix bundle single-stranded DNA binding mode. Based on binding assays and negative-staining electron microscopy results, we found that DnaT can bind to phiX 174 ssDNA to form nucleoprotein filaments for the first time, which indicates that DnaT might function as a scaffold protein during the PriA-dependent primosome assembly. In combination with biochemical analysis, we propose a cooperative mechanism for the binding of DnaT to ssDNA and a possible model for the assembly of PriA-PriB-ssDNA-DnaT complex that sheds light on the function of DnaT during the primosome assembly and stalled replication fork restart. This report presents the first structure of the DnaT C-terminal complex with ssDNA and a novel model that explains the interactions between the three-helix bundle and ssDNA.

PubMed: 25053836
DOI: 10.1093/nar/gku633

実験手法

X-RAY DIFFRACTION (1.96 Å)