4OU3

Crystal structure of porcine aminopeptidase N complexed with CNGRCG tumor-homing peptide

4OU3 の概要

• エントリーDOI: 10.2210/pdb4ou3/pdb
• 関連するPDBエントリー: 4FKE
• 分子名称: Aminopeptidase N, tumor-homing peptide, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: zinc-aminopeptidase, hydrolase-protein binding complex, hydrolase/protein binding
• 由来する生物種: Sus scrofa (pig)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 108994.52

構造登録者

Liu, C.,Yang, Y.,Chen, L.,Lin, Y.-L.,Li, F. (登録日: 2014-02-14, 公開日: 2014-11-05, 最終更新日: 2024-11-27)

主引用文献

Liu, C.,Yang, Y.,Chen, L.,Lin, Y.L.,Li, F.
A Unified Mechanism for Aminopeptidase N-based Tumor Cell Motility and Tumor-homing Therapy.
J.Biol.Chem., 289:34520-34529, 2014

PubMed Abstract: Tumor cell surface aminopeptidase N (APN or CD13) has two puzzling functions unrelated to its enzymatic activity: mediating tumor cell motility and serving as a receptor for tumor-homing peptides (peptides that bring anti-cancer drugs to tumor cells). To investigate APN-based tumor-homing therapy, we determined the crystal structure of APN complexed with a tumor-homing peptide containing a representative Asn-Gly-Arg (NGR) motif. The tumor-homing peptide binds to the APN enzymatic active site, but it resists APN degradation due to a distorted scissile peptide bond. To explore APN-based tumor cell motility, we examined the interactions between APN and extracellular matrix (ECM) proteins. APN binds to, but does not degrade, NGR motifs in ECM proteins that share similar conformations with the NGR motif in the APN-bound tumor-homing peptide. Therefore, APN-based tumor cell motility and tumor-homing therapy rely on a unified mechanism in which both functions are driven by the specific and stable interactions between APN and the NGR motifs in ECM proteins and tumor-homing peptides. This study further implicates APN as an integrin-like molecule that functions broadly in cell motility and adhesion by interacting with its signature NGR motifs in the extracellular environment.

PubMed: 25359769
DOI: 10.1074/jbc.M114.566802

実験手法

X-RAY DIFFRACTION (1.95 Å)