4OTV

Crystal structure of in cellulo Operophtera brumata CPV18

Summary for 4OTV

Entry DOI	10.2210/pdb4otv/pdb
Related	4OTS
Descriptor	Polyhedrin, CHLORIDE ION, GUANOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
Functional Keywords	occlusion body, polyhedra, microcrystals, natural crystal to protect virus, viral protein
Biological source	Operophtera brumata cypovirus 18
Total number of polymer chains	1
Total formula weight	29436.53
Authors	Stuart, D.I.,Sutton, G.C.,Axford, D.,Ji, X. (deposition date: 2014-02-14, release date: 2014-05-14, Last modification date: 2024-02-28)
Primary citation	Axford, D.,Ji, X.,Stuart, D.I.,Sutton, G. In cellulo structure determination of a novel cypovirus polyhedrin. Acta Crystallogr.,Sect.D, 70:1435-1441, 2014 Cited by PubMed Abstract: This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell. PubMed: 24816111 DOI: 10.1107/S1399004714004714 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.7 Å)

Structure validation