4OTV

Crystal structure of in cellulo Operophtera brumata CPV18

4OTV の概要

• エントリーDOI: 10.2210/pdb4otv/pdb
• 関連するPDBエントリー: 4OTS
• 分子名称: Polyhedrin, CHLORIDE ION, GUANOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: occlusion body, polyhedra, microcrystals, natural crystal to protect virus, viral protein
• 由来する生物種: Operophtera brumata cypovirus 18
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29436.53

構造登録者

Stuart, D.I.,Sutton, G.C.,Axford, D.,Ji, X. (登録日: 2014-02-14, 公開日: 2014-05-14, 最終更新日: 2024-02-28)

主引用文献

Axford, D.,Ji, X.,Stuart, D.I.,Sutton, G.
In cellulo structure determination of a novel cypovirus polyhedrin.
Acta Crystallogr.,Sect.D, 70:1435-1441, 2014

PubMed Abstract: This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.

PubMed: 24816111
DOI: 10.1107/S1399004714004714

実験手法

X-RAY DIFFRACTION (1.7 Å)