4OQD

Crystal structure of the tylM1 N,N-dimethyltransferase in complex with SAH and TDP-Qui3NMe2

4OQD の概要

• エントリーDOI: 10.2210/pdb4oqd/pdb
• 関連するPDBエントリー: 4OQE
• 分子名称: dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase, S-ADENOSYL-L-HOMOCYSTEINE, TDP-3,6-dideoxy-3-N,N-dimethylglucose, ... (4 entities in total)
• 機能のキーワード: sam methyltransferase, n-methyltransferase, s-adenosylmethionine, transferase
• 由来する生物種: Streptomyces fradiae
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 117990.78

構造登録者

Thoden, J.B.,Holden, H.M. (登録日: 2014-02-08, 公開日: 2014-02-26, 最終更新日: 2023-09-20)

主引用文献

Thoden, J.B.,Holden, H.M.
Production of a novel N-monomethylated dideoxysugar.
Biochemistry, 53:1105-1107, 2014

PubMed Abstract: The importance of unusual deoxysugars in biology has become increasingly apparent over the past decade. Some, for example, play key roles in the physiological activities of the natural products to which they are attached. Here we describe a study of TylM1, a dimethyltransferase from Streptomyces fradiae involved in the production of dTDP-mycaminose. From this investigation, the manner in which the enzyme binds its dimethylated product has been revealed. More significantly, by providing the enzyme with an alternative substrate, it was possible to produce a monomethylated product not observed in nature. This has important ramifications for the production of unique carbohydrates that may prove useful in drug design.

PubMed: 24512254
DOI: 10.1021/bi500098a

実験手法

X-RAY DIFFRACTION (1.6 Å)