4OOP

Arabidopsis thaliana dUTPase with with magnesium and alpha,beta-imido-dUTP

4OOP の概要

• エントリーDOI: 10.2210/pdb4oop/pdb
• 関連するPDBエントリー: 4OOQ
• 分子名称: Deoxyuridine 5'-triphosphate nucleotidohydrolase, 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: dutpase, hydrolysis, dutp, hydrolase
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 54210.97

構造登録者

Inoguchi, N.,Bajaj, M.,Moriyama, H. (登録日: 2014-02-03, 公開日: 2015-04-08, 最終更新日: 2023-09-20)

主引用文献

Inoguchi, N.,Chaiseeda, K.,Yamanishi, M.,Kim, M.K.,Jang, Y.,Bajaj, M.,Chia, C.P.,Becker, D.F.,Moriyama, H.
Structural insights into the mechanism defining substrate affinity in Arabidopsis thaliana dUTPase: the role of tryptophan 93 in ligand orientation.
BMC Res Notes, 8:784-784, 2015

PubMed Abstract: Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) hydrolyzes dUTP to dUMP and pyrophosphate to maintain the cellular thymine-uracil ratio. dUTPase is also a target for cancer chemotherapy. However, the mechanism defining its substrate affinity remains unclear. Sequence comparisons of various dUTPases revealed that Arabidopsis thaliana dUTPase has a unique tryptophan at position 93, which potentially contributes to its degree of substrate affinity. To better understand the roles of tryptophan 93, A. thaliana dUTPase was studied.

PubMed: 26666293
DOI: 10.1186/s13104-015-1760-1

実験手法

X-RAY DIFFRACTION (1.5 Å)