4OM9

X-Ray Crystal Structure of the passenger domain of Plasmid encoded toxin, an Autrotansporter Enterotoxin from enteroaggregative Escherichia coli (EAEC)

4OM9 の概要

• エントリーDOI: 10.2210/pdb4om9/pdb
• 関連するPDBエントリー: 3SZE
• 分子名称: Serine protease pet (2 entities in total)
• 機能のキーワード: beta-helix, peptidase, alpha-fodrin, hydrolase, eaec, plasmid encoded toxin(pet)
• 由来する生物種: Escherichia coli
• 細胞内の位置: Serine protease pet autotransporter: Periplasm (By similarity). Serine protease pet: Secreted. Serine protease pet translocator: Cell outer membrane; Multi-pass membrane protein (By similarity): O68900
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 104055.56

構造登録者

Meza-Aguilar, J.D.,Fromme, P.,Torres-Larios, A.,Mendoza-Hernandez, G.,Hernandez-Chinas, U.,Arreguin-Espinosa de Los Monteros, R.A.,Eslava-Campos, C.A.,Fromme, R. (登録日: 2014-01-27, 公開日: 2014-03-12, 最終更新日: 2023-09-20)

主引用文献

Domingo Meza-Aguilar, J.,Fromme, P.,Torres-Larios, A.,Mendoza-Hernandez, G.,Hernandez-Chinas, U.,Arreguin-Espinosa de Los Monteros, R.A.,Eslava Campos, C.A.,Fromme, R.
X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC).
Biochem.Biophys.Res.Commun., 445:439-444, 2014

PubMed Abstract: Autotransporters (ATs) represent a superfamily of proteins produced by a variety of pathogenic bacteria, which include the pathogenic groups of Escherichia coli (E. coli) associated with gastrointestinal and urinary tract infections. We present the first X-ray structure of the passenger domain from the Plasmid-encoded toxin (Pet) a 100 kDa protein at 2.3 Å resolution which is a cause of acute diarrhea in both developing and industrialized countries. Pet is a cytoskeleton-altering toxin that induces loss of actin stress fibers. While Pet (pdb code: 4OM9) shows only a sequence identity of 50% compared to the closest related protein sequence, extracellular serine protease plasmid (EspP) the structural features of both proteins are conserved. A closer structural look reveals that Pet contains a β-pleaded sheet at the sequence region of residues 181-190, the corresponding structural domain in EspP consists of a coiled loop. Secondary, the Pet passenger domain features a more pronounced beta sheet between residues 135 and 143 compared to the structure of EspP.

PubMed: 24530907
DOI: 10.1016/j.bbrc.2014.02.016

実験手法

X-RAY DIFFRACTION (2.3 Å)