4OKK

Crystal structure of RNase AS from M tuberculosis in complex with UMP

4OKK の概要

• エントリーDOI: 10.2210/pdb4okk/pdb
• 関連するPDBエントリー: 4oke 4okj
• 分子名称: 3'-5' exoribonuclease Rv2179c/MT2234.1, URIDINE-5'-MONOPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: alpha/beta fold, exoribonuclease, hydrolase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39822.18

構造登録者

Romano, M.,van de Weerd, R.,Brouwer, F.C.C.,Roviello, G.N.,Lacroix, R.,Sparrius, M.,van den Brink-van Stempvoort, G.,Maaskant, J.J.,van der Sar, A.M.,Appelmelk, B.J.,Geurtsen, J.J.,Berisio, R. (登録日: 2014-01-22, 公開日: 2014-05-21, 最終更新日: 2024-03-20)

主引用文献

Romano, M.,van de Weerd, R.,Brouwer, F.C.C.,Roviello, G.N.,Lacroix, R.,Sparrius, M.,van den Brink-van Stempvoort, G.,Maaskant, J.J.,van der Sar, A.M.,Appelmelk, B.J.,Geurtsen, J.J.,Berisio, R.
Structure and Function of RNase AS, a Polyadenylate-Specific Exoribonuclease Affecting Mycobacterial Virulence In Vivo
Structure, 22:719-730, 2014

PubMed Abstract: The cell-envelope of Mycobacterium tuberculosis plays a key role in bacterial virulence and antibiotic resistance. Little is known about the molecular mechanisms of regulation of cell-envelope formation. Here, we elucidate functional and structural properties of RNase AS, which modulates M. tuberculosis cell-envelope properties and strongly impacts bacterial virulence in vivo. The structure of RNase AS reveals a resemblance to RNase T from Escherichia coli, an RNase of the DEDD family involved in RNA maturation. We show that RNase AS acts as a 3'-5'-exoribonuclease that specifically hydrolyzes adenylate-containing RNA sequences. Also, crystal structures of complexes with AMP and UMP reveal the structural basis for the observed enzyme specificity. Notably, RNase AS shows a mechanism of substrate recruitment, based on the recognition of the hydrogen bond donor NH2 group of adenine. Our work opens a field for the design of drugs able to reduce bacterial virulence in vivo.

PubMed: 24704253
DOI: 10.1016/j.str.2014.01.014

実験手法

X-RAY DIFFRACTION (2.21 Å)