4OJI

Crystal Structure of Twister Ribozyme

4OJI の概要

• エントリーDOI: 10.2210/pdb4oji/pdb
• 分子名称: RNA (52-MER), MAGNESIUM ION (3 entities in total)
• 機能のキーワード: pseudoknot, self-cleavage, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17579.03

構造登録者

Liu, Y.,Wilson, T.J.,McPhee, S.A.,Lilley, D.M.J. (登録日: 2014-01-21, 公開日: 2014-07-23, 最終更新日: 2024-02-28)

主引用文献

Liu, Y.,Wilson, T.J.,McPhee, S.A.,Lilley, D.M.
Crystal structure and mechanistic investigation of the twister ribozyme.
Nat.Chem.Biol., 10:739-744, 2014

PubMed Abstract: We present a crystal structure at 2.3-Å resolution of the recently described nucleolytic ribozyme twister. The RNA adopts a previously uncharacterized compact fold based on a double-pseudoknot structure, with the active site at its center. Eight highly conserved nucleobases stabilize the core of the ribozyme through the formation of one Watson-Crick and three noncanonical base pairs, and the highly conserved adenine 3' of the scissile phosphate is bound in the major groove of an adjacent pseudoknot. A strongly conserved guanine nucleobase directs its Watson-Crick edge toward the scissile phosphate in the crystal structure, and mechanistic evidence supports a role for this guanine as either a general base or acid in a concerted, general acid-base-catalyzed cleavage reaction.

PubMed: 25038788
DOI: 10.1038/nchembio.1587

実験手法

X-RAY DIFFRACTION (2.3 Å)