4OJ0

mCardinal V218E

4OJ0 の概要

• エントリーDOI: 10.2210/pdb4oj0/pdb
• 分子名称: Fluorescent protein FP480 (2 entities in total)
• 機能のキーワード: fluorescent protein
• 由来する生物種: Entacmaea quadricolor (Bubble-tip anemone)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 110886.29

構造登録者

Ataie, N.,Ng, H. (登録日: 2014-01-20, 公開日: 2014-03-19, 最終更新日: 2014-05-14)

主引用文献

Chu, J.,Haynes, R.D.,Corbel, S.Y.,Li, P.,Gonzalez-Gonzalez, E.,Burg, J.S.,Ataie, N.J.,Lam, A.J.,Cranfill, P.J.,Baird, M.A.,Davidson, M.W.,Ng, H.L.,Garcia, K.C.,Contag, C.H.,Shen, K.,Blau, H.M.,Lin, M.Z.
Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein.
Nat.Methods, 11:572-578, 2014

PubMed Abstract: A method for non-invasive visualization of genetically labeled cells in animal disease models with micrometer-level resolution would greatly facilitate development of cell-based therapies. Imaging of fluorescent proteins (FPs) using red excitation light in the 'optical window' above 600 nm is one potential method for visualizing implanted cells. However, previous efforts to engineer FPs with peak excitation beyond 600 nm have resulted in undesirable reductions in brightness. Here we report three new red-excitable monomeric FPs obtained by structure-guided mutagenesis of mNeptune. Two of these, mNeptune2 and mNeptune2.5, demonstrate improved maturation and brighter fluorescence than mNeptune, whereas the third, mCardinal, has a red-shifted excitation spectrum without reduction in brightness. We show that mCardinal can be used to non-invasively and longitudinally visualize the differentiation of myoblasts into myocytes in living mice with high anatomical detail.

PubMed: 24633408
DOI: 10.1038/nmeth.2888

実験手法

X-RAY DIFFRACTION (1.7 Å)