4OIO

Crystal structure of Thermus thermophilus pre-insertion substrate complex for de novo transcription initiation

Summary for 4OIO

• Entry DOI: 10.2210/pdb4oio/pdb
• Related: 4OIN 4OIP 4OIQ 4OIR
• Descriptor: DNA-directed RNA polymerase subunit alpha, ADENOSINE-5'-TRIPHOSPHATE, 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine, ... (12 entities in total)
• Functional Keywords: de novo transcription initiation, substrate complex, transcription initiation, i site, i+1 site, rna polymerase, dna/rna/ntp binding, polymerization of ribonucleotides, nucleoid, transcription, transferase
• Biological source: Thermus thermophilus; More
• Cellular location: Cytoplasm (By similarity): Q5SKW1
• Total number of polymer chains: 8
• Total formula weight: 444918.37

Authors

Zhang, Y.,Ebright, R.H.,Arnold, E. (deposition date: 2014-01-20, release date: 2014-05-07, Last modification date: 2023-09-20)

• Primary citation: Zhang, Y.,Degen, D.,Ho, M.X.,Sineva, E.,Ebright, K.Y.,Ebright, Y.W.,Mekler, V.,Vahedian-Movahed, H.,Feng, Y.,Yin, R.,Tuske, S.,Irschik, H.,Jansen, R.,Maffioli, S.,Donadio, S.,Arnold, E.,Ebright, R.H.; GE23077 binds to the RNA polymerase 'i' and 'i+1' sites and prevents the binding of initiating nucleotides.; Elife, 3:e02450-e02450, 2014; PubMed: 24755292

Experimental method

X-RAY DIFFRACTION (3.1 Å)